N5288
Sigma
Anti-NQO1 (C-terminal) antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
| Synonym: | Anti-DIA4, Anti-Diaphorase 4, Anti-DT-diaphorase, Anti-NAD (P)H:Menadione oxidoreductase 1, dioxin-inducible 1, Anti-NAD(P)H dehydrogenase, quinone 1, Anti-NMOR1 |
Description
| Immunogen | synthetic peptide corresponding to amino acid resudues 261-274 of human NQO1 with N-terminal added cysteine conjugated to KLH. The corresponding sequence differs by one amino acid in mouse and rat. |
| Physical form | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
Properties
| antibody form | affinity isolated antibody |
| clone | (polyclonal) |
| form | buffered aqueous solution |
| mol wt | antigen mol wt ~28 kDa |
| species reactivity | human, rat, mouse |
| concentration | ~1 mg/mL |
| application(s) | immunoblotting: 0.1-0.2 μg/mL using whole extract of rat NRK cells |
| immunoblotting: 0.2-0.5 μg/mL using whole extract of mouse 3T3 cells | |
| immunoprecipitation: 2-5 μg using whole extract of human HeLa cells | |
| indirect immunofluorescence: 2-5 μg/mL using human HeLa cells | |
| Gene Information | human ... NQO1(1728) |
| rat ... Nqo1(24314) | |
| mouse ... Nqo1(18104) | |
| shipped in | dry ice |
| storage temp. | −20°C |
